Journal: Biomedicines

Article Title: Epigenetic Regulation of Cardiac Troponin Genes in Pediatric Patients with Heart Failure Supported by Ventricular Assist Device

doi: 10.3390/biomedicines9101409

Figure Lengend Snippet: Experimental design. ( A ) Cardiac samples were collected from HF pediatric patients at the moment of VAD implant (pre-VAD) and at the moment of heart transplant after VAD support (post-VAD). Total RNA (miRNA and mRNA) was extracted from cardiac samples. ( B ) In order to identify the miRNAs modified by VAD support, cardiac miRNA profile at pre-VAD and post-VAD was evaluated by NGS and real-time PCR was performed to validate sequencing results. ( C ) The in-silico analysis was employed to predict the putative targets of validated miRNAs. ( D ) The cardiac expression of miRNAs predicted targets was evaluated in HF children at pre-VAD and post-VAD and compared with a group of adults with HF. ( E ) An in vitro transfection study in HL-1 cell line was performed to verify the regulatory role of selected miRNAs on predicted targets.

Article Snippet: Cardiac Muscle Cell Line (HL-1) (Sigma-Aldrich, St. Louis, MI, USA), an immortalized mouse cardiomyocyte cell line, were grown in 75 cm 2 flasks using Claycomb medium (Sigma-Aldrich, St. Louis, MI, USA), supplemented with 100 μM norepinephrin, 10% fetal bovine serum (FBS) and 4 mM L-glutamine.

Techniques: Modification, Real-time Polymerase Chain Reaction, Sequencing, In Silico, Expressing, In Vitro, Transfection

Journal: Biomedicines

Article Title: Epigenetic Regulation of Cardiac Troponin Genes in Pediatric Patients with Heart Failure Supported by Ventricular Assist Device

doi: 10.3390/biomedicines9101409

Figure Lengend Snippet: In vitro study results. Cardiac troponin C (cTnC) expression levels in HL-1 cell line transfected with ( A ) mimic-miR-1246; ( B ) mimic-19a-3p; ( C ) mimic-199b-5p compared to control (no treated cell). Comparison between control and transfected cells was performed by paired Student’s t-test.

Article Snippet: Cardiac Muscle Cell Line (HL-1) (Sigma-Aldrich, St. Louis, MI, USA), an immortalized mouse cardiomyocyte cell line, were grown in 75 cm 2 flasks using Claycomb medium (Sigma-Aldrich, St. Louis, MI, USA), supplemented with 100 μM norepinephrin, 10% fetal bovine serum (FBS) and 4 mM L-glutamine.

Techniques: In Vitro, Expressing, Transfection

Journal: Cardiovascular Diabetology

Article Title: ZIP7 contributes to the pathogenesis of diabetic cardiomyopathy by suppressing mitophagy in mouse hearts

doi: 10.1186/s12933-024-02499-2

Figure Lengend Snippet: Deficiency of ZIP7 abates T2DM-induced downregulation of mitophagy. A Effect of HG on ZIP7, p62, and LC3 expression levels in AC16 cells. n = 5 experiments per group. B p62, TOM22, TOM20, and VDAC expression levels in mouse hearts. n = 5 mice per group. C LC3-II levels in mitochondria isolated from mouse hearts. n = 4–5 mice per group. D Effects of ZIP7 cKO on mitophagy. Mitophagy were assessed with confocal microscopy in cardiac sections loaded with MitoQC plasmid. n = 5 mice per group. Scale bar, 20 μm. E Confocal images of mouse cardiomyocytes loaded with Keima. n = 4 mice per group. Scale bar, 20 μm. Data are shown in mean ± s.e.m. P values were determined by one-way ANOVA or Student’s t test. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: HL-1 and AC16 cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Isolation, Confocal Microscopy, Plasmid Preparation

Journal: Microorganisms

Article Title: Subspecies Classification and Comparative Genomic Analysis of Lactobacillus kefiranofaciens HL1 and M1 for Potential Niche-Specific Genes and Pathways

doi: 10.3390/microorganisms10081637

Figure Lengend Snippet: Phenotypic characteristics of L. kefiranofaciens strains HL1 and M1. ( A ) Morphology of cells grown in MRS broth; ( B ) Bacterial growth aspect in MRS broth; and ( C ) SDS-PAGE whole-cell protein profiles of four strains of L. kefiranofaciens . The banding patterns were clustered together using the UPGMA algorithm. The evolutionary distances are in the unit of the number of band differences per site, as calculated by p-distance.

Article Snippet: Strain , HL1 , M1 , DSM 10550 T , KR , ATCC 43761 T , ZW3.

Techniques: SDS Page

Journal: Microorganisms

Article Title: Subspecies Classification and Comparative Genomic Analysis of Lactobacillus kefiranofaciens HL1 and M1 for Potential Niche-Specific Genes and Pathways

doi: 10.3390/microorganisms10081637

Figure Lengend Snippet: Differential carbohydrate fermentation characteristics of L. kefiranofaciens strains HL1 and M1, and their closest related type strains by API 50 CHL system. +, Positive; −, negative; w, weakly positive.

Article Snippet: Strain , HL1 , M1 , DSM 10550 T , KR , ATCC 43761 T , ZW3.

Techniques:

Journal: Microorganisms

Article Title: Subspecies Classification and Comparative Genomic Analysis of Lactobacillus kefiranofaciens HL1 and M1 for Potential Niche-Specific Genes and Pathways

doi: 10.3390/microorganisms10081637

Figure Lengend Snippet: Genotypic characterization of L. kefiranofaciens HL1 and M1. ( A ) Phylogenomic tree of L. kefiranofaciens strains using the core gene multilocus sequence typing (cgMLST) of the 1674 core genes, applying the unweighted pair group method with arithmetic mean (UPGMA). Bar, allele numbers. ( B ) The enterobacterial repetitive intergenic consensus (ERIC)-PCR and five randomly amplified polymorphic DNA (RAPD) profiles of L. kefiranofaciens HL1 and M1, as well as reference strains for each strain, were concatenated. The banding patterns were clustered together using the unweighted pair group method with the arithmetic mean (UPGMA) algorithm, using Dice coefficients.

Article Snippet: Strain , HL1 , M1 , DSM 10550 T , KR , ATCC 43761 T , ZW3.

Techniques: Sequencing, Amplification

Journal: Microorganisms

Article Title: Subspecies Classification and Comparative Genomic Analysis of Lactobacillus kefiranofaciens HL1 and M1 for Potential Niche-Specific Genes and Pathways

doi: 10.3390/microorganisms10081637

Figure Lengend Snippet: ( A ) Circular genome maps of L. kefiranofaciens HL1 and M1. The six circles (outer to inner) show the following. Circles 1 and 2 demonstrate the CDSs on the forward and reverse strands, respectively. Circles 3, 4, and 5 show the rRNAs and tRNAs and the GC content. Circle 6 represents the GC skew ((C − G)/(C + G)) curve (positive GC skew, orange; negative GC skew, violet); ( B ) Venn diagrams and Upset plot of coding sequences for the six L. kefiranofaciens strains.

Article Snippet: Strain , HL1 , M1 , DSM 10550 T , KR , ATCC 43761 T , ZW3.

Techniques:

Journal: Microorganisms

Article Title: Subspecies Classification and Comparative Genomic Analysis of Lactobacillus kefiranofaciens HL1 and M1 for Potential Niche-Specific Genes and Pathways

doi: 10.3390/microorganisms10081637

Figure Lengend Snippet: General genomic features of Lactobacillus kefiranofaciens strains.

Article Snippet: Strain , HL1 , M1 , DSM 10550 T , KR , ATCC 43761 T , ZW3.

Techniques: CRISPR

Journal: Microorganisms

Article Title: Subspecies Classification and Comparative Genomic Analysis of Lactobacillus kefiranofaciens HL1 and M1 for Potential Niche-Specific Genes and Pathways

doi: 10.3390/microorganisms10081637

Figure Lengend Snippet: Comparison of genes related to the “Cell Wall and Capsule” SEED Category of L. kefiranofaciens strains.

Article Snippet: Strain , HL1 , M1 , DSM 10550 T , KR , ATCC 43761 T , ZW3.

Techniques: Comparison

Journal: Microorganisms

Article Title: Subspecies Classification and Comparative Genomic Analysis of Lactobacillus kefiranofaciens HL1 and M1 for Potential Niche-Specific Genes and Pathways

doi: 10.3390/microorganisms10081637

Figure Lengend Snippet: Unique genes in L. kefiranofaciens strain HL1, as compared with M1.

Article Snippet: Strain , HL1 , M1 , DSM 10550 T , KR , ATCC 43761 T , ZW3.

Techniques: